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KMID : 0043319990220030267
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Archives of Pharmacal Research
1999 Volume.22 No. 3 p.267 ~ p.273
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Enhanced Macrophage Antitumor Effects of Protein A in Combination with IFN-r
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Pyo Suhk-Neung
Rhee Dong-Kwon
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Abstract
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In this study we examined the potential for the synergistic augmentation of the antitumor activity of inflammatory mouse peritoneal macrophages by stimulation with protein A combined with . The moderate augmentative effect induced by preincubation with protein A was demonstrated to be concentration-dependent, whereas IFN-, had a very low activating effect. Following preincubation with both protein A and , a marked enhancement of macrophage activity was noted. In addition, based on the utilization of neutralizing antibody to TNF- or the inhibition of NO Production, TNF- and NO were proven to be involved as mediators during the activation of tumoricidal macrophages by protein A in combination with . We also demonstrated that supernatants from macrophages treated with protein A plus contained both TNF- and NO at markedly increased levels. Thus, tumor cell lysis in the combined system was mediated via TNF- or NO. These results demonstrate the synergistic effects on mouse pertioneal macrophage function of protein A in combination with and suggest that combinations of such agents may serve as the basis for future in vivo immunotherapy.
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KEYWORD
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Protein A, NO, TNF-α, Marcophage, Antitumor activity
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